منابع مشابه
Quantification and molecular characterization of hprt mutants of human T-lymphocytes.
Somatic mutations have been implicated as critical early events in carcinogenesis. Point mutations, deletions, and translocation events have been shown to activate oncogenes or inactivate suppressor oncogenes. In human population monitoring, quantitative analysis of mutation events that affect gene function is limited to those genes whose cellular phenotypes can be identified by selection proce...
متن کاملMolecular analysis of hprt mutants induced by 2-cyanoethylene oxide in human lymphoblastoid cells.
The mutagenic epoxide metabolite of acrylonitrile, 2-cyanoethylene oxide (ANO), was used to treat human TK6 lymphoblasts (150 microM x 2 h ANO). A collection of hypoxanthine-phosphoribosyltransferase (hprt) mutants was isolated and characterized by dideoxy sequencing of cloned hprt cDNA. Base-pair substitution mutations in the hprt coding region were observed in 19/39 of hprt mutants: 11 occurr...
متن کاملRadioadaptation to the mutagenic effect of ionizing radiation in human lymphoblasts: molecular analysis of HPRT mutants.
A 70% reduction of HPRT- mutant frequency in radioadapted human lymphoblastoid cells has been reported, as analyzed by the Southern blot method (O. Rigaud et al., Radiat. Res., 133: 94-101, 1993). The data reported here extend the previous molecular analysis to a collection of 118 mutants. Structural rearrangements of the HPRT gene were determined using the multiplex polymerase chain reaction a...
متن کاملHprt mutant frequency and molecular analysis of Hprt mutations in Fischer 344 rats treated with thiotepa.
Thiotepa is a bifunctional alkylating anticancer drug that is a rodent carcinogen and a suspected human carcinogen. In order to determine the sensitivity of mutant induction in the Hprt lymphocyte assay for detecting tumorigenic doses of thiotepa, Fischer 344 rats were treated for 4 weeks with thiotepa using a procedure adapted from a carcinogenesis protocol. At various times after beginning th...
متن کاملHPRT: gene structure, expression, and mutation.
INTRODUCTION AND PERSPECTIVES ....................................................... 127 NORMAL ENZYME FUNCTION AND CHARACTERISTICS ............................. 129 Enzymology ........................................................................................ 129 Tissue Distribution ................................................................................ 130 HPRT Gene Structure ....
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ژورنال
عنوان ژورنال: Nature
سال: 1977
ISSN: 0028-0836,1476-4687
DOI: 10.1038/265585a0